SSA could not be possible without the generous support of Drs. Herbert A. and Betty Lou Lubs and the Science, Society, and the Arts Research Conference Endowment. We are deeply grateful!
Over the past few years, the RNA-binding proteins have been increasingly studied. This increase happened after the discovery that the iron-responsive element-binding protein1 (IRP1) is involved in the control of iron metabolism by binding messenger RNA (mRNA) to repress translation or degradation while also participating in the citric acid cycle. Genetic mutations generate dysfunctional metabolic enzymes, being the most common cause of inherited metabolic disorders, such as the hereditary non-spherocytic hemolytic anemia (HNSHA) and the hereditary fructose intolerance (HFI), and also interfering in the RNA-binding process. We study how glycolytic enzymes bind mRNAs, looking at which mRNAs are bound to these enzymes. Our goal is to provide an in vivo description of mRNA targets for glycolytic enzymes, creating a complete picture of mRNA regulation by the glycolytic pathway, as well as to provide new insight into how disease-causing mutations in glycolytic enzymes disrupt RNA-binding.
