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SSA could not be possible without the generous support of Drs. Herbert A. and Betty Lou Lubs and the Science, Society, and the Arts Research Conference Endowment. We are deeply grateful!
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Friday, March 17 • 3:00pm - 4:15pm
Easel 13: Analysis of gene expression in a female rat model of diet-induced non-alcoholic fatty liver disease using RNA-sequencing

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We have established a diet-induced model of non-alcoholic fatty liver disease (NAFLD) in the adult female rat, which we have characterized through RNA-seq, histology, and physiological measurements. Furthermore, we investigated whether peripheral estrogen signaling has affected this disease. We observed a significant effect of both diet and estrogen on animal weight gain. Our high-fat, high-sugar animals demonstrate numerous physiological and histological markers of NAFLD. Additionally, we used RNA purified from liver tissues to quantify global changes in gene expression between our experimental groups through RNA-seq, with the goal of identifying genes that are differentially expressed in the NAFLD induced animals. Interestingly we see the greatest effect on gene expression through the interaction between diet and estrogen. We will be discussing the in-depth, high resolution gene analysis done on the liver tissues as it relates to the induction of this disease.


Friday March 17, 2017 3:00pm - 4:15pm
Leyburn Library